Peptide Synthesis Procedures

1. Peptide synthesizer - Wang Resin
2. General procedures for manual synthesis - with permission from Nowick Lab, UCI

Material Safety Data Sheets (MSDS) for commonly used chemicals:

1. Dimethylformamide (DMF)
2. Dichloromethane (DCM)
3. Piperidine
4. Ethyl cyanohydroxyiminoacetate (Oxyma)
5. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)
6. N,N'-Diisopropylcarbodiimide (DIC)
7. HATU
8. HBTU
9. HCTU
10. HOAt
11. HBPyU
12. BOP
13. PyBOP
14. Hexamethylphosphoramide (HMPA) - Side product with BOP
15. Tetramethylurea (TMU) - Side product with HATU, HBTU, HCTU
16. Trifluoroacetic acid (TFA)
17. 2,2'-(Ethylenedioxy)diethanethiol (DODT)
18. Triisoproylsilane (TIS)
19. 2,4,6-Trimethylpyridine (Collidine)
20. N,N-Diisopropylethylamine (DIPEA)
21. N-methylmorpholine
22. Hexafluoroisopropanol (HFIP)
23. Fmoc-Ala-OH
24. Fmoc-Arg(Pbf)-OH
25. Fmoc-Asn(Trt)-OH
26. Fmoc-Asp(OtBu)-OH
27. Fmoc-Cys(Trt)-OH
28. Fmoc-Gln(Trt)-OH
29. Fmoc-Glu(OtBu)-OH
30. Fmoc-Gly-OH
31. Fmoc-His(Trt)-OH
32. Fmoc-Ile-OH
33. Fmoc-Leu-OH
34. Fmoc-Lys(Boc)-OH
35. Fmoc-Met-OH
36. Fmoc-Phe-OH
37. Fmoc-Pro-OH
38. Fmoc-Ser(tBu)-OH
39. Fmoc-Thr(tBu)-OH
40. Fmoc-Trp(Boc)-OH
41. Fmoc-Tyr(tBu)-OH
42. Fmoc-Val-OH